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Structured Review

Staples mutations in cep290
<t>Cep290</t> recruitment to centrosomal satellites and basal bodies depends on SSX2IP in RPE-1 cells. (a) Cells were transfected with control or SSX2IP siRNA and stained for Cep290 and γ-tubulin using indirect immunofluorescence. (b) Average projection of 50 cells immunostained as shown in a. Centering of single images before projection was based on the γ-tubulin signal. (c, d) Quantification of signal intensities of Cep290 at the basal body (c) or in satellites (d) of cells transfected with control or SSX2IP siRNA. (c, d) Left (bars), mean values of averages ± SEM from three independent experiments ( n = 150) normalized to controls. Right (box-and-whiskers plots), quantification of a single representative experiment. * p < 0.05, ** p < 0.01, *** p < 0.001 (two-tailed Student's t test).
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1) Product Images from "The novel centriolar satellite protein SSX2IP targets Cep290 to the ciliary transition zone"

Article Title: The novel centriolar satellite protein SSX2IP targets Cep290 to the ciliary transition zone

Journal: Molecular Biology of the Cell

doi: 10.1091/mbc.E13-09-0526

Cep290 recruitment to centrosomal satellites and basal bodies depends on SSX2IP in RPE-1 cells. (a) Cells were transfected with control or SSX2IP siRNA and stained for Cep290 and γ-tubulin using indirect immunofluorescence. (b) Average projection of 50 cells immunostained as shown in a. Centering of single images before projection was based on the γ-tubulin signal. (c, d) Quantification of signal intensities of Cep290 at the basal body (c) or in satellites (d) of cells transfected with control or SSX2IP siRNA. (c, d) Left (bars), mean values of averages ± SEM from three independent experiments ( n = 150) normalized to controls. Right (box-and-whiskers plots), quantification of a single representative experiment. * p < 0.05, ** p < 0.01, *** p < 0.001 (two-tailed Student's t test).
Figure Legend Snippet: Cep290 recruitment to centrosomal satellites and basal bodies depends on SSX2IP in RPE-1 cells. (a) Cells were transfected with control or SSX2IP siRNA and stained for Cep290 and γ-tubulin using indirect immunofluorescence. (b) Average projection of 50 cells immunostained as shown in a. Centering of single images before projection was based on the γ-tubulin signal. (c, d) Quantification of signal intensities of Cep290 at the basal body (c) or in satellites (d) of cells transfected with control or SSX2IP siRNA. (c, d) Left (bars), mean values of averages ± SEM from three independent experiments ( n = 150) normalized to controls. Right (box-and-whiskers plots), quantification of a single representative experiment. * p < 0.05, ** p < 0.01, *** p < 0.001 (two-tailed Student's t test).

Techniques Used: Transfection, Control, Staining, Immunofluorescence, Two Tailed Test

Related Articles

Transfection:

Article Title: The novel centriolar satellite protein SSX2IP targets Cep290 to the ciliary transition zone
Article Snippet: Of importance, mutations in Cep290, also known as BBS14, were previously shown to correlate with several ciliopathies, including Bardet–Biedl syndrome, Meckel–Gruber syndrome, and Joubert syndrome ( Staples et al. , 2012 ).

Control:

Article Title: The novel centriolar satellite protein SSX2IP targets Cep290 to the ciliary transition zone
Article Snippet: Of importance, mutations in Cep290, also known as BBS14, were previously shown to correlate with several ciliopathies, including Bardet–Biedl syndrome, Meckel–Gruber syndrome, and Joubert syndrome ( Staples et al. , 2012 ).

Staining:

Article Title: The novel centriolar satellite protein SSX2IP targets Cep290 to the ciliary transition zone
Article Snippet: Of importance, mutations in Cep290, also known as BBS14, were previously shown to correlate with several ciliopathies, including Bardet–Biedl syndrome, Meckel–Gruber syndrome, and Joubert syndrome ( Staples et al. , 2012 ).

Immunofluorescence:

Article Title: The novel centriolar satellite protein SSX2IP targets Cep290 to the ciliary transition zone
Article Snippet: Of importance, mutations in Cep290, also known as BBS14, were previously shown to correlate with several ciliopathies, including Bardet–Biedl syndrome, Meckel–Gruber syndrome, and Joubert syndrome ( Staples et al. , 2012 ).

Two Tailed Test:

Article Title: The novel centriolar satellite protein SSX2IP targets Cep290 to the ciliary transition zone
Article Snippet: Of importance, mutations in Cep290, also known as BBS14, were previously shown to correlate with several ciliopathies, including Bardet–Biedl syndrome, Meckel–Gruber syndrome, and Joubert syndrome ( Staples et al. , 2012 ).

Membrane:

Article Title: The novel centriolar satellite protein SSX2IP targets Cep290 to the ciliary transition zone
Article Snippet: Of importance, mutations in Cep290, also known as BBS14, were previously shown to correlate with several ciliopathies, including Bardet–Biedl syndrome, Meckel–Gruber syndrome, and Joubert syndrome ( Staples et al. , 2012 ).



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Staples mutations in cep290
<t>Cep290</t> recruitment to centrosomal satellites and basal bodies depends on SSX2IP in RPE-1 cells. (a) Cells were transfected with control or SSX2IP siRNA and stained for Cep290 and γ-tubulin using indirect immunofluorescence. (b) Average projection of 50 cells immunostained as shown in a. Centering of single images before projection was based on the γ-tubulin signal. (c, d) Quantification of signal intensities of Cep290 at the basal body (c) or in satellites (d) of cells transfected with control or SSX2IP siRNA. (c, d) Left (bars), mean values of averages ± SEM from three independent experiments ( n = 150) normalized to controls. Right (box-and-whiskers plots), quantification of a single representative experiment. * p < 0.05, ** p < 0.01, *** p < 0.001 (two-tailed Student's t test).
Mutations In Cep290, supplied by Staples, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mutations+in+cep290/cep290+protein/pmc03923641-131-4-26
Average 90 stars, based on 1 article reviews
mutations in cep290 - by Bioz Stars, 2026-10
90/100 stars
  Buy from Supplier

Image Search Results


Cep290 recruitment to centrosomal satellites and basal bodies depends on SSX2IP in RPE-1 cells. (a) Cells were transfected with control or SSX2IP siRNA and stained for Cep290 and γ-tubulin using indirect immunofluorescence. (b) Average projection of 50 cells immunostained as shown in a. Centering of single images before projection was based on the γ-tubulin signal. (c, d) Quantification of signal intensities of Cep290 at the basal body (c) or in satellites (d) of cells transfected with control or SSX2IP siRNA. (c, d) Left (bars), mean values of averages ± SEM from three independent experiments ( n = 150) normalized to controls. Right (box-and-whiskers plots), quantification of a single representative experiment. * p < 0.05, ** p < 0.01, *** p < 0.001 (two-tailed Student's t test).

Journal: Molecular Biology of the Cell

Article Title: The novel centriolar satellite protein SSX2IP targets Cep290 to the ciliary transition zone

doi: 10.1091/mbc.E13-09-0526

Figure Lengend Snippet: Cep290 recruitment to centrosomal satellites and basal bodies depends on SSX2IP in RPE-1 cells. (a) Cells were transfected with control or SSX2IP siRNA and stained for Cep290 and γ-tubulin using indirect immunofluorescence. (b) Average projection of 50 cells immunostained as shown in a. Centering of single images before projection was based on the γ-tubulin signal. (c, d) Quantification of signal intensities of Cep290 at the basal body (c) or in satellites (d) of cells transfected with control or SSX2IP siRNA. (c, d) Left (bars), mean values of averages ± SEM from three independent experiments ( n = 150) normalized to controls. Right (box-and-whiskers plots), quantification of a single representative experiment. * p < 0.05, ** p < 0.01, *** p < 0.001 (two-tailed Student's t test).

Article Snippet: Of importance, mutations in Cep290, also known as BBS14, were previously shown to correlate with several ciliopathies, including Bardet–Biedl syndrome, Meckel–Gruber syndrome, and Joubert syndrome ( Staples et al. , 2012 ).

Techniques: Transfection, Control, Staining, Immunofluorescence, Two Tailed Test